Juan S. Bonifacino, Mary Dasso, Joe B. Harford, Jennifer Lippincott-Schwartz, Kenneth M. Yamada (eds.) «Current Protocols in Cell Biology»
Current Protocols in Cell Biology | ISBN: 0471241083 | 2004 Year | PDF | 41 Mb | 3178 Pages
DOI: 10.1002/0471143030.cbfores00
Developed by leading scientists in the field, Current Protocols in Cell Biology is an essential reference for researchers who study the relationship between specific molecules and genes and their location, function and structure at the cellular level.
* includes nearly 1,000 detailed protocols carefully written by the foremost investigators in cell biology from all around the world.
* offers expert research tips and guidelines for isolating and culturing cells in two- or three-dimensional cultures, analyzing the morphology of the cell, and characterizing the functions of organelles and molecules in the cell and its environment.
* includes special appendices on the use of inhibitors and pharmacologic agents, protein motifs, absorption and emission maxima for common fluorophores, and more.
Current Protocols in Cell Biology provides methods for basic culture and fractionation of cells; microscopic localization and tracking; and analysis of biosynthesis, modification, and transport of specific molecules. In addition, the one-volume looseleaf manual offers more complex procedures involved in the determination of the role of intracellular and extracellular environment; molecular reconstitution of specific processes; molecular and temporal analysis of the cell cycle; investigation of intracellular and intercellular communications; and analysis of intact cells, organelles, and specific molecules. These topics are thoroughly explained with detailed protocols, critical parameters and troubleshooting guidelines. Extensive background information and authors' commentaries provide all the necessary information and guidance to facilitate successful results. Quarterly updates, filed into the looseleaf binder, keep you and your laboratory current with the latest developments in cell biology methodology.